Bartonella henselae & HIF-1



Arbeitsgruppe Prof. Dr. med. Volkhard A. J. Kempf
e-mail: volkhard.kempf(at)kgu.de





 

Bartonella henselae

Bartonella henselae, the agent of cat-scratch disease in immunocompetent individuals, is a fastidious facultative intracellular, gram-negative pathogen. It is also the causal agent of bacillary angiomatosis and bacillary peliosis, which are histologically characterized as lobulated proliferation of endothelial cells in immunocompromised patients (e.g. AIDS).

Endothelial cells are one presumed habit at of Bartonella spp.The ability to induce vasculoproliferative disorders in humans is a unique feature of Bartonella spp., however the underlying molecular mechanisms need to be further elucidated. Parts of our current projects are described below; our research is funded by the Deutsche Forschungsgemeinschaft (SFB 766 and priotity research programs 1130, 1131, 1316).

Vasculoproliferative factors in Bartonella infections

The research group 'Bartonella' is investigating host cell-interactions mainly of Bartonella henselae. A novel 'two-step' model of pathogenicity has been described suggesting that host cell-derived vasculoproliferative factors play a crucial role in the pathogenesis of bacillary angiomatosis ("paracrine-loop model"). The resulting proliferation of endothelial cells could be interpreted as bacterial pathogens triggering the promotion of their own habitat: the host cell. Similar disease mechanisms are well known in the plant pathogen Agrobacterium tumefaciens which causes crown gall disease.
This fascinating angiogenic potential is of particular interest in terms of analysing bacteria-triggered tumour formation. Solid-tumour growth in animals and plants depends on the formation of a sophisticated vascular network consisting of blood vessels or water-conducting elements, respectively. The elucidation of tumourous diseases caused by bacteria might provide new insights into microbial pathogenicity, tumour growth and pathological angiogenesis. Moreover, the idea of the induction of a complete neoangiogenic process by a bacterium or its recombinant products is a fascinating concept in terms of the development of novel treatment strategies of ischemic disorders such as artherosclerosis.

Trimeric autotransporter adhesins (TAA)

We identified a nonfimbrial adhesin of B. henselae designated as Bartonella adhesin A (BadA). BadA is a 340-kD outer membrane protein, has a trimeric structure and contains domains homologous to the Yersinia enterocolitica adhesin A (YadA) and to the Xanthomonas adhesin-like protein A (XadA) of the plant pathogen Xanthomonas oryzae pv. oryzae.

Together these TAAs belong to the recently described family of trimeric autotransporter adhesins (TAA), which are important virulence factors of Gram-negative bacteria. BadA mediates the binding of B. henselae to extracellular matrix proteins and to endothelial cells, possibly via beta1-integrins, but prevents phagocytosis. Expression of BadA iscrucial for activation of hypoxia-inducible factor (HIF)-1 in host cells by B. henselae and secretion of proangiogenic cytokines (e.g., vascular endothelial growth factor,VEGF). BadA is immunodominant in B. henselae-infected patients and rodents, indicating that it is expressed during Bartonella infections. Our results suggest that BadA, the largest characterized bacterial protein thus far, is a major pathogenicity factor of B. henselae with a potential role in the induction of vasculoproliferative disorders. Currently, there are several projects ongoing aimed at the exact identification of BadA domain-function relationships.

The role of hypoxia-inducible factor (HIF)-1 in infections

HIF-1 has been identified as the key transcription factor involved in hypoxia adaptive processes and angiogenesis. However, there is increasing evidence that HIF-1 (in addition to its role in angiogenesis and cancer) may also be involved in inflammatory and infectious diseases. At present, these novel aspects of HIF-1 function are only poorly understood. Therefore, we suggest an essential role of HIF-1 in infections with human pathogens. Indeed, results revealed HIF-1 recent induced gene programming as excellent ta